Optimizing RNAi for Cell Culture
Tuesday, July 13, 2010, 12:00 PM Eastern Time (ET)

RNA interference (RNAi) is a powerful tool that can regulate the expression of genes through double stranded RNA (dsRNA). This methodology of gene expression is sequence-specific as the dsRNA silences the corresponding endogenous messenger RNA (mRNA). Although the technique was first discovered in Caenorhabditis elegans, its utilization has been applied to a wide range of organisms. More importantly, RNAi can be readily performed with cells in culture using various mechanisms to understand the function of specific genes. We will present several common mechanisms typically used for the study or RNAi with cells in culture and highlight important factors to consider to achieve maximum results.

Speaker:

John Shyu, Ph.D.

John Shyu, Ph.D., is a Field Application Scientist for Corning Life Sciences. He received his doctorate degree from Purdue University in the area of gene expression and regulation using various model organisms and techniques. Also, he developed and optimized several in vivo protein-protein detection assays using bimolecular fluorescence complementation (BiFC).

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